Light-switching of Enzymatic Activity in Functionalized Polymer Brushes


UV- and visible light-induced switching of enzymatic activity has been demonstrated using surface-grafted polymer brushes functionalized with microperoxidase MP-11 and spiropyran mojeties. Integration into an optofluidic device allowed reversible switching of the enzymatic activity under flow.

Copolymer brushes, composed of glycidyl methacrylate and a furan-protected maleimide-containing monomer, were grafted from radical initiators at the surface of irradiation-activated fluoropolymer foils. After postpolymerization modification with enzymatically active microperoxidase-11 and photochromic spiropyran moieties, the polymer brushes catalyzed the oxidation of 3,3’5,5’-tetramethylbenzidine. Exposure to either UV or visible light allowed switching the turnover by more than one order of magnitude, as a consequence of the reversible, light-induced spiropyran-merocyanine transition.

Light-Switching of Enzymatic Activity  
(a) Strategy for orthogonal functionalization of polymer brushes using specific amine-apoxide and thiol-ene coupling reactions. (b,c) Demonstration of the enzymatic activity and (d,e) of the UV-light induced change in wettability.
Light-Switching of Enzymatic Activity  
(a) Schematic illustration of the fabricated optofluidic device integrated with the modified ETFE substrate. (b) Measurement of the enzymatic activity before (orange) and after (purple) exposure to UV light: Three consecutive cycles were measured with 60 minutes relaxation intervals.
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Tue Jul 25 16:36:24 CEST 2017
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