Light-switching of Enzymatic Activity in Functionalized Polymer Brushes
UV- and visible light-induced switching of enzymatic activity has been demonstrated using surface-grafted polymer brushes functionalized with microperoxidase MP-11 and spiropyran mojeties. Integration into an optofluidic device allowed reversible switching of the enzymatic activity under flow.
Copolymer brushes, composed of glycidyl methacrylate and a furan-protected maleimide-containing monomer, were grafted from radical initiators at the surface of irradiation-activated fluoropolymer foils. After postpolymerization modification with enzymatically active microperoxidase-11 and photochromic spiropyran moieties, the polymer brushes catalyzed the oxidation of 3,3’5,5’-tetramethylbenzidine. Exposure to either UV or visible light allowed switching the turnover by more than one order of magnitude, as a consequence of the reversible, light-induced spiropyran-merocyanine transition.
- Matthias Dübner
- Nicholas D. Spencer
- Laboratory for Surface Science & Technology
- Reversible Light-Switching of Enzymatic Activity on Orthogonally Functionalized Polymer Brushes
Matthias Dübner, Victor J. Cadarso, Tugce N. Gevrek, Amitav Sanyal, Nicholas D. Spencer, and Celestino Padeste
ACS Appl. Mater. Interfaces 2017, in press. DOI: 10.1021/acsami.7b01154